The session will be mainly focused on sharing our experience of inadvertently handling level 3 organisms in a high-volume regional level 2 laboratory in Southern Ontario. The session will present interesting cases of Brucella with unusual presentation in blood cultures. Similarly a case of slow growing fastidious Gram negative coccobacilli which was identified as Francisella tularensis from lymph node biopsy culture, a fluffy dimorphic fungus “Coccidiodes immitis/posadesii” from lung tissue culture in absence of pertinent clinical history of suspicious organism and a exposure to non-fastidious, rapidly growing level 3 organism “Burkholderia pseudomallei ” will be dealt in brief. We will share our prevention strategies for dealing with these organisms and outline a process for risk mitigation after possible exposure.